rRT-PCR is performed by almost all state and local public health laboratories, the APHL Vaccine Preventable Disease Reference Centers (VPD-RC ), and CDC. The trend was reversed for IgM detection with increasing time to specimen collection the number of seropositive specimens increased.Ī: Viral detection methods include molecular assays, such as real time reverse transcription PCR (rRT-PCR) to detect mumps viral RNA. ![]() ![]() In an outbreak setting (see Figure below), the proportion of specimen with positive results by rRT-PCR (N target gene) decreased as the interval between onset and specimen collection increased (Rota et al., 2013). ![]() If the patient has orchitis/oophoritis, mastitis, pancreatitis, hearing loss, meningitis, or encephalitis, collect a buccal swab specimen for rRT-PCR and a serum specimen for IgM detection, regardless of days since symptom onset. If it has been 3 days since symptom onset, collect a buccal swab specimen for rRT- PCR and a serum specimen for IgM detection. The early collection of buccal swab specimens provides the best means of laboratory confirmation, particularly among suspected mumps patients with a history of vaccination (Rota et al, 2013). ![]() A: A buccal swab specimen for detection of virus should be collected as soon as possible upon suspicion of mumps disease.
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